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Image Search Results
Journal: Frontiers in Physiology
Article Title: Axial Tubule Junctions Activate Atrial Ca 2+ Release Across Species
doi: 10.3389/fphys.2018.01227
Figure Lengend Snippet: Atrial expression of Ca 2+ handling proteins in different rodent species, pig, and human. (A) Immunoblots comparing the expression of ryanodine receptor 2 (RyR2), sodium-calcium exchanger 1 (NCX), SR Ca 2+ -ATPase 2 (SERCA2), sodium-potassium ATPase α1 subunit (Na,K), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), phospholamban (PLN), and phospholemman (PLM). Insets document the intensity-corrected rabbit NCX and SERCA2 as used for quantification (arrowheads) and additional rabbit-specific antibody cross-reactions (asterisks). Also, the PLN pentameric (p) and monomeric (m) bands are indicated. See Supplementary Figure for full blots and Supplementary Table for clinical patient information. Immunoblot/GAPDH signals (B) and relative protein ratios (C) each normalized to human. M, mouse; Rt, rat; Rb, rabbit; P, pig; H, human. Student’s t -test ∗ P < 0.05, ∗∗ P < 0.01.
Article Snippet: Membranes were blocked for 1 h in 5% (w/v) non-fat milk in Tris-buffered saline with 0.05% (v/v) Tween 20, and incubated with the primary antibodies overnight at 4°C as follows: RyR2 1:2500 (HPA020028, Sigma-Aldrich); NCX 1:1000 (11-13, Swant);
Techniques: Expressing, Western Blot